Advances in Applied Microbiology, Vol. 3 by Wayne W Umbreit

By Wayne W Umbreit

Meant for researchers in utilized microbiology and environmental engineers, this publication covers such themes as environmental evaluation of biotechnological tactics and microbial adjustments of haloaromatic and haloaliphatic compounds.

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Pyogenes before lyophilization, more bacilli seemed to survive than when glucose was present, although the latter markedly increased survival above that obtained in the absence of sugars. Survival during storage of lyophilized cultures of P. , 1958) containing glucose was no better than the control containing no additive, whereas, in the presence of either lactose or sucrose, organisms survived 20 months' storage at room temperature. Similarly Scott ( 1958); after testing cultures of Salmonella newport lyophilized in sucrose, glucose, and arabinose, found the latter to be the least effective.

G. EXTENTOF DRYING Most researchers agree that residual moisture content is a factor influencing the survival of organisms but there are diverse opinions as to what the optimal moisture content should be for optimal survival. It has been repeatedly stated, mostly without any evidence, that cultures should be as dry as possible to preserve viability, the reasoning being that only in the absence of water is the metabolism of the organism completely arrested. Others believe, however, that it is possible to dry organisms excessively and that one should allow a certain amount of moisture to remain.

20OC. 37°C. 0001 - 0 4°C. 37°C. 37°C. 25% thiourea 4°C. 50% Skim milk and phosphate buffer 2"-4"C. 7 10 Years Growth - References __ Alexander and van Drimmelen (1956) and van Drimmelen (1956) Heckly et al. (1960) Hornibrook (1950) - Hutton et al. (1951) Rhoades (1958) 26 Weeks 4gL van Drimmelen and Steyn (1958) 50 45 17 Verwey (1945) Rr 0 19 Months 19 Months RT 3 Years - 0 20 Days 84 Days 84 Days 56 Days 84 Days __ 3°C. 3°C. 3°C. 3°C. 3°C. 00015 100 Heller (1941) 50 33 PRESERVATION OF BACTERIA BY LYOPHILIZATION TABLE I (Continued) Storage Organism and suspending fluid Temperature Time 5 % Lactose plus salts Mist,.

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