By Allen I. Laskin
An eclectic quantity of topical studies on all features of utilized microbiology. It comprises 14 accomplished studies of present examine in utilized microbiology.
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M. (1992). The rapid generation of mutation data matrices from protein sequences. Comput. Appl. Biosci. 8, 275–282. , Borchert, T. , and Fuglsand, C. C. (2002). Industrial enzyme applications. Curr. Opin. Biotechnol. 13, 1–7. Lange, C. , Wackett, L. , and Daly, M. (1998). Engineering a recombinant Deinococcus radiodurans for organopollutant degradation in radioactive mixed waste environments. Nat. Biotechnol. 16, 929–933. , and Wandrey, C. (2000). ’’ WileyVCH, Weinheim, Germany. , and Daniel, R.
As a reporter in gene fusion constructs. , 2000). , 1959) and is widely used as a reporter in whole-cell biosensors. Its enzyme product -galactosidase normally cleaves the disaccharide lactose into glucose and galactose. However, it can also cleave commercially developed substrates such as X-gal to produce a blue pigment that is detectable spectrophotometrically. It is a relatively easy, reliable, and quick reporter assay to apply to whole-cell biosensors but as described for xylE, its main disadvantage is its widespread presence in bacteria.
1997). ’’ Wiley-VCH, Weinheim, Germany. Cottrell, M. , Moore, A. , and Kirchman, D. L. (1999). Chitinases from uncultured marine microorganisms. Appl. Environ. Microbiol. 65, 2553–2557. , and Almatawah, Q. (1998). Biochemistry and biotechnology of mesophilic and thermophilic nitrile metabolizing enzymes. Extremophiles 2, 207–216. , Nyandorah, M. , Bull, A. , and Hardman, D. J. (1997). Some biochemical properties and the classification of a range of bacterial haloalkane dehalogenases. Biotechnol.