Advances in Applied Microbiology, Vol. 52 by Allen I. Laskin

By Allen I. Laskin

An eclectic quantity of topical studies on all features of utilized microbiology. It comprises 14 accomplished studies of present examine in utilized microbiology.

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M. (1992). The rapid generation of mutation data matrices from protein sequences. Comput. Appl. Biosci. 8, 275–282. , Borchert, T. , and Fuglsand, C. C. (2002). Industrial enzyme applications. Curr. Opin. Biotechnol. 13, 1–7. Lange, C. , Wackett, L. , and Daly, M. (1998). Engineering a recombinant Deinococcus radiodurans for organopollutant degradation in radioactive mixed waste environments. Nat. Biotechnol. 16, 929–933. , and Wandrey, C. (2000). ’’ WileyVCH, Weinheim, Germany. , and Daniel, R.

As a reporter in gene fusion constructs. , 2000). , 1959) and is widely used as a reporter in whole-cell biosensors. Its enzyme product -galactosidase normally cleaves the disaccharide lactose into glucose and galactose. However, it can also cleave commercially developed substrates such as X-gal to produce a blue pigment that is detectable spectrophotometrically. It is a relatively easy, reliable, and quick reporter assay to apply to whole-cell biosensors but as described for xylE, its main disadvantage is its widespread presence in bacteria.

1997). ’’ Wiley-VCH, Weinheim, Germany. Cottrell, M. , Moore, A. , and Kirchman, D. L. (1999). Chitinases from uncultured marine microorganisms. Appl. Environ. Microbiol. 65, 2553–2557. , and Almatawah, Q. (1998). Biochemistry and biotechnology of mesophilic and thermophilic nitrile metabolizing enzymes. Extremophiles 2, 207–216. , Nyandorah, M. , Bull, A. , and Hardman, D. J. (1997). Some biochemical properties and the classification of a range of bacterial haloalkane dehalogenases. Biotechnol.

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